In this paper the results of the in vitro study of synthesized derivatives 1,2,3-triazolo-1,3,4-thiadiazine on morpho- and rhizogenesis, as well as tuberization of microplants of the “Irbitskiy” potato variety are described. It has been shown that the use of compounds of this series is very promising for acceleration of growth and development of explants. In this study, substance T6 showed the best results. By the number of rudiments of tubers on the stolons, plants grown on nutrient media with T3 were prominent, however, by quality indicators for nutrient media with T6 was better. 1,2,3-Triazolo-1,3,4-thiadazine T6, which showed the most pronounced results, is recommended for further study of the possibility of its use in potato production.

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