Fish species of the Centropomus genus are important food resources in Brazil. The species of this group are predators that feed on small fish and benthic invertebrates, and are therefore vulnerable to bioconcentration and the biomagnification of contaminants. As estuaries are continuously impacted by urbanization, the aim of the present study was to evaluate the quality of two brackish environments located in the Ilha do Maranhão, Brazil, by applying the Micronucleus Test with Centropomus undecimalis. Genotoxic lesions were detected in the erythrocytes using this test. Blood samples were collected through punctures of the gills to produce the blood extensions. The smear was stained with modified Rosenfield dye and 2,000 erythrocytes per fish were analyzed. The micronucleus values differed significantly between the two locations in the rainy season, showing that Centropomus undecimalis is more vulnerable to contamination in the Bacanga River Basin. In view of the results, the Micronucleus Test proved to be an effective tool for the evaluation of the environmental quality of areas, and the data generated in the study can contribute to conservation programs by local management authorities, as the biomarker used allows the evaluation of the health of the environment through two organisms.

1.
Arslan
,
O. C.
;
Boyacioglu
,
M.
;
Parlak
,
H.
;
Katalay
,
S.
;
Karaaslan
,
M. A.
Assessment of micronuclei induction in peripheral blood and gill cells of some fish species from Aliağa Bay Turkey
”.
Marine Pollution Bulletin
,
94
:
48
54
(
2015
).
2.
Carrano
,
A. V.
;
Nataranjan
,
S.
Considerations for populations monitoring using cytogenetic techniques
”.
Mutation Research
, v.
204
, p.
379
406
(
2002
).
3.
Carrard
,
V. C.
,
Ferreira
,
L. A.
,
Lauxen
,
I. D. S.
,
Costa
,
C. H.
,
Rados
,
P. V.
Teste dos Micronúcleos: Um biomarcador de dano genotóxico em células descamadas da mucosa bucal
”.
Revista da Faculdade de Odontologia de Porto Alegre.
V.
48
, n.
1/3
, p.
77
81
(
2007
).
4.
Fenech
,
M.
The in vitro micronucleus technique
”.
Mutation Research
, v.
445
, p.
81
95
, (
2000
).
5.
Fenech
,
M.
;
Cheng
,
W. P.
;
Kirsch-Volders
,
M.
;
Holland
,
N.
;
Bonassi
,
S.
;
Zeiger
,
E.
Humn project: “
Detailed description of the scoring criteria for the 49 cytokinesis-block micronucleus assay using isolated human lymphocyte cultures
”.
Mutation Research
, v.
534
, p.
65
75
, (
2003
).
6.
Ferraro
,
M. V. M.
;
Fenocchio
,
A. S.
;
Mantovani
,
M. S.
;
Ribeiro
,
C. O.
;
Cestari
,
M. M.
Mutagenic effects of tributyltin and inorganic lead (Pb II) on the fish H. malabaricus as evaluated using the comet assay and the piscine micronucleus and chromosome aberration tests
”.
Genetics and Molecular Biology
.
27
, n.
1
, p.
103
107
, (
2004
).
7.
Kirschbaum
,
A. A.
;
Seriani
,
R.
;
Pereira
,
C. D. S.
;
Assunção
,
A.
;
Abessa
,
D. M. S.
;
Rotundo
,
M. M.
;
Ranzani-Paiva
,
M. J. T.
Cytogenotoxicity biomarkers in fat snook Centropomus parallelus from Cananéia and São Vicente estuaries, SP, Brazil
”.
Genetics and Molecular Biology
, v.
32
, n.
1
, p.
151
154
, (
2009
).
8.
Martinez
,
C.B.R.
;
Cólus
,
I.M.S.
Biomarcadores em peixes neotropicais para o monitoramento da poluição aquática na bacia do rio Tibagi
”. In:
A bacia do rio Tibagi
, cap.
29
, p.
551
557
, (
2002
).
9.
Mateuca
,
R.
,
Lombaert
,
N.
,
Aka
,
P. V.
,
Decordier
,
I.
Kirsch Volders
,
M.
Chromosomal changes: induction, detection methods and applicability in human biomonitoring
”.
Facets of environmental nuclear toxicology
, v.
88
, p.
1515
1531
,
2006
.
10.
Matsumoto
,
S. T
;
Mantovani
,
M. S
;
Malaguttii
,
M. I.A
;
Dias
,
A. L
;
Fonseca
,
I. C
;
Marin-Morales
,
M. A.
Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Orechromis niloticus and chromossome aberrations in on root-tips
”.
Genetics and Molecular Biology
.
29
, n.
1
, p.
148
158
, (
2006
).
11.
Obiakor
,
M. O.
;
Okonkwo
,
J. C.
;
Nnabude
,
P. C.
Ezeonyejiaku
,
C. D.
Eco-genotoxicology: Micronucleus Assay in Fish Erythrocytes as In situ Aquatic Pollution Biomarker: a Review
”.
Journal of Animal Science Advances
,
2
(
1
):
123
133
(
2012
).
12.
Ramirez
,
A.
;
Saldanha
,
P.H.
Micronucleus Investigation of Alcoholic Patients with Oral Carcinomas
”.
Genetics and Molecular Biology
, Ribeirão Preto, v.
1
, no.
3
, p.
246
260
(
2002
).
13.
Ranzani-Paiva
,
M.J.T.
; et al. 
Métodos para análise hematológica em peixes
.
Maringá: Eduem
,
2013
,
140p
.
14.
Serrano-Garcia
,
L.
;
Monteromontoya
,
R.
“Micronuclei and Chromatid Buds are the Result of Related Genotoxic Events”.
Environmental and Molecular Mutagenesis
,
New York
, v.
38
, no.
1
, p.
38
45
, July
2001
.
15.
Smith
,
K. L.
;
Guentzel
,
J. L.
Mercury concentrations and omega-3 fatty acids in fish and shrimp: preferential consumption for maximum health benefits
”.
Marine Pollution Bulletin
, n.
60
, p.
1615
1618
(
2010
).
16.
Souza
,
I. C.
;
Duarte
,
I. A. N. D.
;
Pimentel
,
N. Q.
;
Rocha
,
L. D.
;
Morozesk
,
M.
;
Bonomo
,
M. M.
;
Azevedo
,
V. C.
;
Pereira
,
C. D. S.
;
Monferrán
,
M. V.
;
Milanez
,
C. R. D.
;
Matsumoto
,
S. T.
;
Wunderlin
,
D. A.
;
Fernandes
,
M. N.
Matching metal pollution with bioavailability, bioaccumulation and biomarkers response in fish (Centropomus parallelus) resident in neotropical estuaries
”.
Environmental Pollution
, v.
180
, p.
136
144
, (
2013
).
17.
Turkmen
,
M.
;
Turkmen
,
A.
;
Tepe
,
Y.
;
Tore
,
Y.
;
Ates
,
A.
Determination of metals in fish species from Aegean and Mediterranean Seas
”.
Food Chemistry
, v.
113
, p.
233
237
(
2009
).
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