After each harvesting season, rice straws are disposed via open burning which caused air pollution. To reduce the air pollution caused by open burning, bio valorisation of rice straw via solid state fermentation was carried in this research to biosynthesis schizophyllan. Isolate UTAR A1 was identified as Schizophyllum commune UTARA1 based on its 18S rDNA. From the preliminary screening, the results showed that rice straw is a promissing biomass for the synthesis of schizophyllan and its maximum production was observed on Day 19. Design Expert® 7.0.0 was used to screen for the variables that affect the production of schizophyllan. Based on the analysis of variance (ANOVA), the model terms are significant as the p-value is less than 0.05. Among the parameter tested, weight of substrate (p-value 0.0021) has a negative impact on the production, while there was correlation between inoculum size and moisture to substrate ratio with a p-value of 0.0249. In conclusion, this research shows there were correlation between factors in this optimization of schizophyllan production research via the 2-level factorial design.

Topics
Biomass energy sources, Air pollution, Educational assessment, Biosynthesis, Fermentation process, Analysis of variance
1.
A.
 
John
,
Agric. Food Policy Stud. Institute, Malaysia.
 
18
, (
2013
), pp.
1
–
5
.
Google Scholar
 
2.
B.
 
Gadde
,
C.
 
Menke
 and
R.
 
Wassmann
,
Biomass and Bioenergy.
 
33
, (
2009
), pp.
1532
–
1546
.
https://doi.org/10.1016/j.biombioe.2009.07.018
Google Scholar
Crossref
Search ADS
 
3.
M. Z.
 
Rosmiza
,
W. P.
 
Davies
,
C. R. R.
 
Aznie
,
M.
 
Mazdi
 and
M. J.
 
Jabil
,
Geogr. Malaysian J. Soc. Sp.
 
10
, (
2014
), pp.
30
–
43
.
Google Scholar
 
4.
Urmila
,
Int. J. Humanit. Soc. Sci. Res.
 
3
, (
2017
), pp.
16
–
19
.
Google Scholar
 
5.
M.
 
Kumari
,
S. A.
 
Survase
 and
R. S.
 
Singhal
,
Bioresour. Technol.
 
99
, (
2008
), pp.
1036
–
1043
.
https://doi.org/10.1016/j.biortech.2007.02.029
Google Scholar
Crossref
Search ADS
PubMed
 
6.
Y.
 
Zhang
,
H.
 
Kong
,
Y.
 
Fang
,
K.
 
Nishinari
 and
G. O.
 
Phillips
,
Bioact. Carbohydrates Diet. Fibre.
 
1
, (
2013
), pp.
53
–
71
.
https://doi.org/10.1016/j.bcdf.2013.01.002
Google Scholar
Crossref
Search ADS
 
7.
Y. C.
 
Sim
, “
Molecular identification of Schizophyllum commune and its production of schizophyllan
,”
Universiti Tunku Abdul Rahman.
(
2014
).
Google Scholar
 
8.
E.
 
Smit
,
P.
 
Leeflang
,
B.
 
Glandorf
,
J. D
 
van Elsas
 and
K.
 
Wernars
,
Society.
 
65
, (
1999
), pp.
2614
–
2621
.
Google Scholar
 
9.
J.
 
Wu
,
X.
 
Wu
,
L.
 
Li
,
R.
 
Zhang
,
Q
,
Dai
,
L
,
Zhao
 and
J.
 
Li
, China patent 104316481 (
2015
).
10.
P.
 
Yadav
 and
R.
 
Tyagi
,
Experimental Biotechnology
. (
Discovery Publishing House
,
New Delhi
,
2005
).
Google Scholar
 
11.
G.
 
Walsh
,
Proteins: Biochemistry and Biotechnology
. (
John Wiley and Sons
,
New York
,
2002
).
Google Scholar
 
12.
E.
 
Holtzman
 and
Lysosomes
. (
Springer Science and Business Media
,
Berlin
,
2013
).
Google Scholar
 
13.
R.G.
 
Reyes
,
W.
 
Graßl
 and
U.
 
Rau
,
J. Nature Studies
,
7
(
2009
).
Google Scholar
 
14.
J.
 
Nitschke
,
H.
 
Modick
,
E.
 
Busch
,
R.W.
 
von Rekowski
,
H. J.
 
Altenbach
 and
H.
 
Mölleken
,
Food Chemistry
,
127
, (
2011
), pp.
791
–
96
.
https://doi.org/10.1016/j.foodchem.2010.12.149
Google Scholar
Crossref
Search ADS
PubMed
 
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