Chalcones have been widely researched its activity as anticancer. The substitution of the functional groups on the chalcones structure affects its anticancer activity. The objective of this research were to investigate the cytotoxic effects of bromo chalcone [1-(4’-bromophenyl)-3-(4-hydroxy-3-methoxyphenyl)-2-propene-1-on] or BHM on T47D breast cancer cells by invitro, and to analyze its effect on cell apoptosis. Cytotoxic activity of BHM on T47D cells was assayed by MTT (3-[4, 5-dimethylthiazol-2-yl)-2, 5 diphenyl tetrazolium bromide] method, while the effect of apoptosis was analyzed using flowcytometer. Furthermore, the apoptotic mechanism was traced by observing the expression of Bcl-2 protein using immunocytochemistry. The results indicated that BHM have cytotoxic effect on T47D cells (IC50 = 45 micro molar). The activity occurs because these compounds induce apoptosis by decreasing the expression of Bcl-2. According to the results, BHM could potentially be developed as a new drug candidate for breast cancer therapy.

Topics
Inorganic compounds, Diseases and conditions, Apoptosis, Proteins, Cancer treatment
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