Fortification of natural folic acid powder and its identification in preparation of infant biscuit for complementary feeding

Powder of mixtures of tempeh, fermented vegetables and nixtamalized corn (Zea mays var.identata) have potential use as fortificant of source of natural folic acid. Its application in preparation of infant biscuit as Complementary Feeding was conducted to achieve folic acid for infant growth and development. This experiment activity aims to find out the best biscuit characteristic on composition, domination of folic acid monomer, distribution of particles size, and type of volatile compounds. The experiment activity was performed on 2 (two) type of fortificants, namely powder of mixtures of nixtamalized yellow corn and soy (G. soyae) tempeh with fermented broccoli (Brassica oleracea L.), (fortificant A) and fermented spinach (Amaranthus sp.) (fortificant B) with adding folic acid at concentrations of 0, 200, 400, 600 and 800 µg/basic formula weight of biscuit, respectively. The results of experiment showed that optimization of the best biscuit formula by adding fortificant A and fortificant B was achieved with fortificant concentration 800 µg/145 g of basic formula and gave compositions of folic acid 127.23 and 117.38 µg/mL, dissolved protein 2.18 and 2.76 mg/mL, total solids93.46 and 93.48 %, total sugars 348.7 and 313.31 mg/mL, and reducing sugars 47.53 and 51.61 mg/mL. Besides, they gave 11 and 7 folic acid monomers dominated by vanillin (46.055 and 50.410 %), furan (6.910 and 4.035 %), fatty acids (38.12 and 18.84 %), ester (3.656 and 8.464 %), and distribution of particles size displayed particle size 405.3 nm and 315.8 nm with Index particle (IP) 0.736 and 0.438.