Morphological changes on development of Tenebrio molitor L. (Coleoptera: Tenebrionidae) in rearing room system, free air CO₂ enrichment system and open roof ventilation system

Tenebrio molitor known as darkling beetle is burrowing insects and can be found all over the world. Tenebrio molitor samples were put in Open Roof Ventilation System, Free Air CO₂ Enrichment and Rearing Room system. The development of Tenebrio molitor adult parents (P) and first generation (F1) that more exposed to high level of CO₂ were studied. As the results, parent and first generation (F1) of T. molitor shows that there are no significant effects of CO₂ on their development. But, decreased development pattern showed between parent and F1 can be used to estimate that prolonged exposure of CO₂ will change their development. The highest development of parent showed by OFVSP (0.1708±0.0013) mm, while the lowest showed by FACEP (0.1686±0.0013) mm. For F1, the highest development showed by OFVS F1 (0.1705±0.0015) mm, while RR F1 becomes the lowest (0.1649±0.0023) mm. No significant difference shown by ANOVA test. Unfortunately, the data cannot be used to prove that the development of parent and F1 were affected by CO₂ because no significant difference recorded between them. Correlation coefficient analysis of OFVSP and OFVS F1 presented to support the results. It shows that the correlation coefficient among characters from parents and F1 are different. Further study should be done in future to increase our knowledge of the climate change on insects.