Every company in Indonesia is responsible for their worker health and safety condition as mentioned in UU No I year 1970. In manufacturing industries, there are many manual tasks dealing with high work load and risk, so that they require excellent concentration and physical condition. There is no ideal way to guarantee worker safety without a real time physiological monitoring. This paper reports our ongoing study in conceptual design development of worker’s clothing which is equipped with a wearable instrumentation system. The system is designed to detect and measure body temperature and pulse in real time. Some electrical components such as, LCD (liquid crystal display), LEDs (light emitting diode), batteries, and physiological sensors were assembled. All components are controlled by a wearable on board controller. LEDs is used as alert which can indicate abnormal physical conditions. The LCD was added to provide more detail information. TMP 36 and XD-58C were selected as the physiological sensors. Finally, an Arduino Lilypad was chosen for the controller. This instrumentation system was verified by accurately detected and inform physiological condition of 3 subjects. Further we are going to attach the system to a worker’s clothing which was specifically designed to simplify and comfortable usage.

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