The objective of this research was to get the mechanism of nano size chitosan particle growth during storage by observing the effect of temperature and initial concentration of chitosan. The products were analyzed using PSA to have the average of particle radius. Nanochitosan solution was prepared by ionic gelation method. This method is described as an electrostatic interaction between positively charged amine with negatively charged polyanion, such as tripolyphosphate (TPP). Chitosan was dissolved in 1% acetic acid and was stirred for 30 minutes. Tween 80 was added to avoid agglomeration. TPP was prepared by dissolving 0.336 g into distilled water. The nano size chitosan was obtained by mixing TPP and chitosan solution dropwise while stirring for 30 minutes. This step was done at 15°C and ambient temperature (about 30°C) and chitosan concentration 0.2%, 0.4% and 0.6%. The results show that temperature during ionic gelation process (15°C and 30°C) does not affect the initial size of the nanoparticles produced as well as the growth of the nanoparticles during storage. On the other hand, initial chitosan concentration strongly affects initial size of the nanoparticles produced and the growth of the nanoparticles during storage. The concentration of chitosan at 0.2%, 0.4%, 0.6% gave initial size of nanoparticle chitosan of 175.3 nm, 337.9 nm, 643.3 nm respectively. On the other hand, the growth mechanism of chitosan nanoparticle depended on its radius(R). At R<500 nm, the growth rate of nanoparticles is controlled by adsorption at the surface of the particles, while at R>500 nm, it is controlled by diffusion in the liquid film around the particles.

1.
Fadli
R.
,
2015
,
Limbah Kitin Yang Bernilai Tambah
,
Direktorat Jenderal Pengolahan dan Pemasaran Hasil Perikanan
,
Kementerian Kelautan dan Perikanan RI
.
2.
Fardiaz
,
S.
,
1989
,
Mikrobiologi Pangan
,
Bogor
:
PAU Pangan dan Gizi IPB.
3.
Illum
,
L.
,
1998
,
Chitosan and Its Use as a Pharmaceufical Excipient
,
Pharmaceutical research
, Vol.
15
. No.
9
.
1326
1331
.
4.
Komariah
,
A.
,
2014
,
Staphylococcus aureus (in vitro) Antibacterial Activity of Nano-Chitosan on Staphylococcus aureus
,
Seminar Nasional XI Pendidikan Biologi FKIP UNS Biologi.
371
377
.
5.
Mardliyati
,
E.
,
Muttaqien
,
E.S.
,
Setyawati
,
D.R.
,
2012
,
Sintesis Nanopartikel Kitosan-Trypolyphosphate dengan Metode Gelasi Ionik : Pengaruh Konsentrasi dan Rasio Volume Terhadap Karakteristik Partikel
,
Serpong
:
Prosiding Pertemuan Ilmiah Ilmu Pengetahuan dan Teknolog Bahan.
6.
Tiyaboonchai
,
W.
,
2003
,
Chitosan Nanoparticles: A Promising System For Drug Delivery
,
Naresuan University J.
,
11
(
3
).
51
66
.
7.
Tsai
,
M.L.
,
Chen
,
R.H.
,
Bai
,
S.W.
,
Chen
,
W.Y.
,
2011
,
The Storage Stability of Chitosan / Tripolyphosphate Nanoparticles in a Phosphate Buffer
,
Taiwan
:
National Taiwan Ocean University.
8.
Uragami
,
T.
,
Tokura
,
S.
,
2006
,
Material Science of Chitin and Chitosan
,
Tokyo
:
Kodansha, Ltd.
9.
Wen
,
T.
,
Brush
,
L.N.
,
Krishnan
,
K.M.
,
2014
,
A Generalized Diffusion Model for Growth of Nanoparticles Synthesized by Colloidal Methods
, Seattle:
Journal of Colloid and Interface Science
,
419
.
79
85
.
10.
Xu
,
Y.
,
Du
,
Y.
,
2003
,
Effect of Moleculer Structure of Chitosan on Protein Delivery Properties of Chitosan Nanoparticles
,
International Journal of Pharmaceutics
,
250
.
215
226
.
11.
Zhao
,
L.
,
Shi
,
L.
,
Zhang
,
Z.
,
Chen
,
J.
,
Shi
,
D.
,
Yang
,
J.
,
Tang
,
Z.
,
2011
,
Preparation and Application of Chitosan Nanoparticles and Nanofibers
,
28
(
03
).
353
362
.
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