Peroxidases (EC: 1.11.1.7) are haem proteins and contain iron (III) protoporphyrin IX (ferriprotoporphyrin IX) as the prosthetic group [1]. They are found in all cells and play a critical role in many biological processes, such as the host-defense mechanism [2]. Peroxidases (PODs) are widely used in clinical biochemistry, enzyme immunoassays, synthesis of various aromatic chemicals, treatment of waste water containing phenolic compounds [3, 4]. In this study, peroxidase enzyme was purified with Para amino benzohydrazide (PABH)-L-Tyrosine Sepharose 4B affinity chromatography to investigate the inhibitory effect of hydrazide derivatives on Turnip (Brassica rapa L.). IC50 values and Ki constants were calculated for the molecules of 6-Amino nicotinic hydrazide, 6-Amino-5-bromo nicotinic hydrazide, 2-Amino-5-hydroxy benzohydrazide, 4-Amino-3-hydroxy benzohydrazide on purified enzyme and inhibition type of these molecules were determined.
Skip Nav Destination
Article navigation
25 April 2017
II. INTERNATIONAL CONFERENCE ON ADVANCES IN NATURAL AND APPLIED SCIENCES: ICANAS 2017
18–21 April 2017
Antalya, Turkey
Research Article|
April 25 2017
Investigation of hydrazide derivatives inhibitory effect on peroxidase enzyme purified from turnip roots
Züleyha Almaz;
Züleyha Almaz
a)
1
Mus Alparslan University
Faculty of Arts and Sciences, Department of Molecular Biology and Genetics, 49250-Mus, Turkey
Search for other works by this author on:
Aykut Öztekіn;
Aykut Öztekіn
b)
2
Agri Ibrahim Cecen University
Vocational School of Health Services, Department of Medical Services and Techniques, 04100-Agri, Turkey
Search for other works by this author on:
Hasan Özdemіr
Hasan Özdemіr
c)
3
Ataturk University
, Science Faculty, Department of Chemistry, 25240-Erzurum, Turkey
Search for other works by this author on:
AIP Conf. Proc. 1833, 020095 (2017)
Citation
Züleyha Almaz, Aykut Öztekіn, Hasan Özdemіr; Investigation of hydrazide derivatives inhibitory effect on peroxidase enzyme purified from turnip roots. AIP Conf. Proc. 25 April 2017; 1833 (1): 020095. https://doi.org/10.1063/1.4981743
Download citation file:
Pay-Per-View Access
$40.00
Sign In
You could not be signed in. Please check your credentials and make sure you have an active account and try again.
43
Views
Citing articles via
Inkjet- and flextrail-printing of silicon polymer-based inks for local passivating contacts
Zohreh Kiaee, Andreas Lösel, et al.
Effect of coupling agent type on the self-cleaning and anti-reflective behaviour of advance nanocoating for PV panels application
Taha Tareq Mohammed, Hadia Kadhim Judran, et al.
Design of a 100 MW solar power plant on wetland in Bangladesh
Apu Kowsar, Sumon Chandra Debnath, et al.
Related Content
Determining inhibition effects of some aromatic compounds on peroxidase enzyme purified from white and red cabbage
AIP Conference Proceedings (April 2016)
Results of breeding of spring rapeseed and spring turnip rape in Western Siberia
AIP Conf. Proc. (June 2023)
The results of breeding of winter turnip rape in V.S. Pustovoit All-Russian Research Institute of Oil Crops
AIP Conf. Proc. (June 2023)
Synthesis and characterization of novel benzohydrazide as potential antibacterial agents from natural product vanillin and wintergreen oil
AIP Conference Proceedings (March 2017)
Clay modification by epoxidized fatty hydrazides synthesized from palm oil
AIP Conf. Proc. (February 2019)