Microchannel heat sink creates an innovative cooling technology to remove large amount of heat from small area. Recently, nanotechnology gain interest to explore the microchannel cooling benefits of nanofluids as working fluid. The objective of this study is to investigate the effect of heat transfer to Al2O3 nanofluids after used as working fluid in the microchannel. In this study, the microchannel was design in square shape with a cross section of 0.5×0.5 mm2 and made by copper. The experiment was conducted in laminar flow with Reynolds number ranging approximately from 633 to 1172. The present study was focused on heat transfer of Al2O3 nanofluids in microchannel heat sink at concentration of 1.0 wt. % and 2.5 wt. % dispersed in water. The heat was produced at bottom of the heat sink is 325 W. The computational simulation method was carried out to validate the experimental results. It was observed that the heat transfer rate is higher when using Al2O3 nanofluids compared to water. However, according to X-ray diffraction method (XRD), it is found that the structure of Al2O3 particles tends to more integrity and the crystallite size grows up after increased the temperature in the microchannel.

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