The study was conducted to determine the anti-hyperglycemic property in terms of α-glucosidase inhibitory activity of the various parts (corm, leaf and petiole) of Colocasia esculenta (L.) Schott var. PSB-VG #9. Each of the plant parts were extracted with 95% ethanol and concentrated using a rotary evaporator at 40 °C. The crude extracts were screened for the presence of alkaloids, flavonoids, glycosides and saponins using Thin Layer Chromatography. The α-glucosidase inhibitory activity of the crude extracts (50 mg/L) were assayed spectrophotometrically using a microplate reader. The results of the phytochemical screening revealed the presence of alkaloids, flavonoids, and saponins in the leaf part while flavonoids and saponins were detected in the petiole and only saponins were present in the corm. The assay showed that the percentage α-glucosidase inhibition of the 50 mg/L ethanolic crude extract of the corm, leaves and petiole of C. esculenta are 68.03, 71.64 and 71.39%, respectively. Statistical analysis shows significant differences in the α-glucosidase inhibition among the various plant parts. It can be concluded that the ethanolic crude extracts of the different parts of C. esculenta (L.) Schott var. PSB-VG #9 exhibited inhibitory activity against α-glucosidase and the presence of phytochemicals like alkaloids, flavonoids and saponins may have contributed greatly to the inhibitory activity of the plant extract and can be further subjected for isolation of the therapeutically active compounds with antidiabetes potency.

1.
T. O.
Ijaola
,
A.A
Osunkiyesi
,
A. A.
Taiwo
,
O. A.
Oseni
,
Y. A.
Lanreiyanda
,
J. O.
Ajayi
and
R. T.
Oyede
,
Journal of Applied Chemistry.
7
(
5
),
16
25
(
2014
).
2.
S. M
Ahmed
,
S. B.
Vrushhabendra
,
P. R. R.
Dhanapal
and
V.
Chandrashheraka
,
Iranian Journal of Pharmacology & Therapeutics.
4
,
36
39
(
2005
).
3.
V.
Ghadyale
,
S.
Takalikar
,
V.
Haldavnekar
and
A.
Arvindekar
,
Evidence-Based Complementary and Alternative Medicine.
2012
,
372909
(
2012
).
4.
D. M.
Nathan
,
N Engl J Med.
356
,
437
440
(
2007
).
5.
V.
Ghadyale
,
S.
Takalikar
,
V.
Haldavnekar
and
A.
Arvindekar
,
Evidence-Based Complementary and Alternative Medicine.
2012
,
372909
(
2012
).
6.
S.
Kumar
,
S.
Narwal
,
V.
Kumar
and
O.
Prakash
,
Pharmacognosy Reviews.
5
(
9
),
19
29
(
2011
).
7.
R.
Tunis
,
M. R.
Loizze
and
P.
Menichini
,
Mini Reviews in Medicinal Chemistry.
10
(
4
),
315
331
(
2010
).
8.
D. S. L.
Cliede
,
P. L. D.
Joao
,
A. P.
Bruno
,
A. P. G. E. F.
Rozilaine
,
A. N.
Manoel
,
S. A.
Renata
,
G. R. Q.
Maria
,
C. F. S.
Francisca
, and
S. B. V.
Glauce
,
Biology Pharmacy Bulletin.
21
(
1
),
125
127
(
2004
).
9.
E. M.
Halim
,
Indian Journal Clinical Biochemistry.
18
,
54
63
(
2003
).
10.
C. J.
Bailey
and
C.
Day
,
Diabetes Care.
12
,
553
564
(
1989
).
11.
N. S.
Kumawat
,
S. P.
Chaudhari
,
N. S.
Wani
,
T. A.
Deshmukh
, and
V. R.
Patil
,
International Journal of PharmTech Research.
2
(
2
),
1246
1249
(
2010
).
12.
H.
Wagner
and
S.
Bladt
,
Plant Drug Analysis: A Thin Layer Chromatography Atlas
(
Springer-Verlag
,
New York
,
1996
), pp.
99
101
.
13.
J.
Senguttuvan
and
S.
Paulsamy
,
PharmTech Res.
4
(
2
),
147
(
2014
).
14.
Y. M.
Kim
,
Y. K.
Jeong
,
H.
Wang
,
W. Y.
Lee
, and
H. I.
Rhee
,
Nutrition.
21
(
6
),
756
761
(
2005
).
15.
T. O.
Nakayama
,
T. J.
Harvey
and
C. K.
Tsung Hui
,
Journal of Food Science.
42
,
2123
2126
(
2006
).
16.
C. P.
Khare
,
Indian Medicinal Plant: An Illustrated Dictionary
(
Springer Science and Business Media
,
New York
,
2007
), p.
207
.
17.
C. N. A.
Leong
, “
Studies on the chemical characteristics of flavonoids glycosides isolated from subtropical plants in Okinawa and their biological activities
”, Ph.D. thesis,
Kagashima University
,
2010
.
18.
R. M.
Alcantara
,
W. A.
Hurtada
and
E. I.
Dizon
,
J. Nutr Food Sci.
3
,
207
(
2013
).
19.
K. C.
Ong
and
H. E.
Khoo
,
Life Science.
67
(
14
),
1695
705
(
2000
).
This content is only available via PDF.
You do not currently have access to this content.