Fermentation on spinach (Amaranthus sp.) vegetable by kombucha culture as an effort to get poliphenol as antioxidant compound had been done. Purification of fermented spinach extract suspension was carried out through microfiltration (MF) membrane (pore size 0.15 µm) fitted in dead-end Stirred Ultrafiltration Cell (SUFC) mode at fixed condition (stirrer rotation 400 rpm, room temperature, pressure 40 psia). Result of the experimental activity showed that long fermentation time increased total acids, total polyphenol and Total Plate Count (TPC), and decreased total solids and reducing sugar in biomass. The optimal fermentation time was reached for 2 weeks with total polyphenol recovery increasing of 92.76 % from before and after fermentation. On this optimal fermentation time, biomass had identified galic acid with relative intensity of 8 %, while as polyphenol monomer was resulted 5 kinds of polyphenol compounds with total intensity 27.97 % and molecular weight (MW) 191.1736, 193.1871 and 194.2170 at T2.5, T2.86 and T3.86. Long fermentation time increased functional properties of polyphenol as antioxidant.

1.
Malbasa
,
R.
,
Loncar
,
E.
and
Djuric
,
M.
2008
. “
Comparison of the products of Kombucha fermentation on sucrose and molasses
”,
Journal of Food Chemistry
106
:
1039
1045
, (2008).
2.
Jayabalan
,
R.
,
Subathradevi
,
Marimuthu
,
S.
,
Sathshkumar
,
M.
And
Swaminathan
,
K
, “
Changes in free-radical scavenging ability of kombucha tea during fermentation
”.
Food Chem.
2008,
109
(
1
) pp.
227
234
(
2008
).
3.
Z.
Liu
, “New techniques for tea catechins extraction,” in
International Training Workshop of Tea Science
(
Hunan Agricultural University
.
China
,
21
July-10 August
2006
).
4.
Anonymous
.
Membrane Technology For Process Industry
,(http:www.pcims.com./images/TP105.5us.pdf;
PCI Membrane System Inc.
Milford, USA
,
2005
).
5.
A. S.
Michael
,.
Handbook of Industrial Membrane Technology
, (
Noyes Publications
,
Park Ridge, U. S. A.
,
1989
)
6.
D.
Onggo
, “
General Principles in Electrospray Mass Spectrometry: A New Technique in Mass Spectral Analysis
”,
Journal JMS
3
(
2
),
115
131
(
1998
).
7.
P.
Eichhorn
and
T. P.
Knepper
, 2001 “
Electrospray ionization mass spectrometric studies on the amphoteric surfactant cocamidopropylbetaine
”,
Journal of Mass Spectrometry
36
,
677
684
(
2011
).
8.
AOA
,.
Official Methods of Analysis
, (
Association of Official Analytical Chemists
,
Washington, D. C
,
1995
)
9.
Anonymous
,
Catalogue and Product Information of Stirred Ultrafiltration Cell
, (
Amicon Bioseparation
,
MILLIPORE, Bedford, U. S. A
,
2002
)
10.
H. D.
Belitz
and
W.
Grosch
,
Food Chemistry
, 2nd Edition (
Springer-Verlag
,
Berlin Heidelberg, Germany
,
2002
).
11.
Anonymous
.
Kombucha
, (Wikipedia Indonesia. Available from : http://en.wikipedia.org/wiki/Kombucha, accessed 10 March 2008).
12.
Scott
Keith
.
Handbook of Industrial Membranes
, 2nd. Edition,
Elsevier Advanced Technology
,
Oxford
,
763
768
, (
1998
)
This content is only available via PDF.
You do not currently have access to this content.