Orthosiphon stamineus Benth (Lamiaceae) is a plant with many ethnobotanical uses including antifungal and antibacterial activities. This study is aimed to determine the cytotoxicity and phytochemical content of O. stamineus leaves and flower using ethanol and water as solvents. The cytotoxicity of the extracts towards Vero cell was determined by MTT assay. The CC50 values were between 3.4-7.4 mg/ml and can be considered as nontoxic. Phytochemical screening revealed terpenes, alkaloid and phenolic were present in the leaves and flower of O. stamineus that might pose as the bioactive compound.
REFERENCES
1.
L.
Van-Buren
, C.I.
Kroner
, M.M.G.
Koning
. J. Ethnopharmacol.
114
, 1
–31
(2007
).2.
PT Eisai
Indonesia
, (Ed.), 1995
. Medicinal Herb Index in Indonesia
, second ed., PT Eisai Indonesia
, Indonesia
.3.
H.
Muhammad
, M.R.
Gomes-Carneirob
and A.
Afzanc
. J. Ethnopharmacol.
133
(2
), 154
–158
(2009
).4.
S.
Awale
, Y.
Tezuka
, A. H.
Banskota
, and S.
Kadota
. Bio. Pharmbull.
26
(4
), 468
–473
(2003
).5.
S.
Awale
, Y.
Tezuka
, A. H.
Banskota
, K.
Kouda
, M. T.
Kyaw
, and S.
Kadota
. Plant Med.
6
(3
), 286
–288
(2002
).6.
F.
Amal
, I.
Nazlina
and W.A.
Yaacob
. Am. J. Drug Discov. Dev.
3
(2
), 84
–94
(2013
).7.
J. B.
Harborne
, Phytochemical Methods: A Guide To Modern Techniques Of Plant Analysis
, 2nd Edition, Chapman And Hall Publications
, London, New York
8.
G. A.
Akowuah
, Z.
Ismail
, I.
Norhayati
and A
, Sadikun
. Food Chem.
93
(2
), 311
–317
(2005
).9.
S. C.
Gad
, In Vitro Toxicology
. 2nd Edition, Taylor & Francis
, Philadelphia, PA
. (2000
).10.
M.
Malahubban
, A. R
Alimon
, A. Q.
Sazili
, S.
Fakurazi
and F. A.
Zakry
, Trop. Biomed.
30
, 467
–480
(2013
).11.
O. Z.
Ameer
, I. M.
Salman
, M. Z.
Asmawi
, Z. O.
Ibraheem
, M. F.
Yam
. J. Med. Food.
15
(8
), 678
–690
(2012
).12.
T.
Hussain
, S.
Fareed
, H. H.
Siddiqui
, M.
Vijaykumar
and C. V
Rao
. Asian Pac. J. Trop. Dis.
2
(2
), 129
–132
(2012
).13.
M. R.
Kumbhare
, V.
Guleha
and T.
Sivakumar
. Asian Pac. J. Trop. Dis.
2
(2
), 144
–150
(2012
).14.
T.
Nobori
, K.
Miurak
, Wu
, D.J.
, Takabayashik
, L.A.
and Carson
, D.A.
1994
. Nature
46
: 753
–756
.15.
M. J.
Just
, M. C.
Recio
, R. M.
Giner
, M. U.
Cueller
, S.
Manez
, A.R.
Billia
and J.L
Rios
. Plant Med.
64
, 404
–407
(1998
).16.
17.
18.
A.
Ali
, M.
Mackeen
, M. M.
El-Sharkawy
, S. H.
Abdul Hamid
, J.
Ismail
, N. H.
Ahmad
, F., & Lajis
, M. N.
(1996
). Pertanika. J. Trop. Agr. Sci.
19
(2/3
), 129
–136
This content is only available via PDF.
© 2015 AIP Publishing LLC.
2015
AIP Publishing LLC
You do not currently have access to this content.