This research will discuss the design of electroporation generator using Arduino as the pulse controller. The pulse parameters are the most important thing in electroporation method, therefore many researches aimed to produce generator to control its parameters easily. Arduino will be used as the microcontroller to create low amplitude signal trigger to get the high voltage pulse for electroporation. 124.4 VDC will be used and tested in cuvette contained NaCl solution with various concentration between 0% - 1%.
REFERENCE
1.
Beebe
, S.J.
, et al., Non-ionizing radiation with nanosecond pulsed electric fields as a cancer treatment: in vitro studies
. Conf Proc IEEE Eng Med Biol Soc
, 2009
. 2009
: p. 6509
–12
.2.
Beebe
, S.J.
, N.M.
Sain
, and W.
Ren
, Induction of cell death mechanisms and apoptosis by nanosecond pulsed electric fields (nsPEFs)
. Cells
, 2013
. 2
(1
): p. 136
–162
.3.
Grenier
, J.R.
, Design of a MOSFET-Based Pulsed Power Supply for Electroporation
. 2006
: University of Waterloo
.4.
Baker
, R.
and B.
Johnson
, A 500 V nanosecond pulse generator using cascode-connected power MOSFETs
. Measurement Science and Technology
, 1992
. 3
(8
): p. 775
.5.
Baker
, R.
and S.
Ward
, Designing nanosecond high voltage pulse generators using power MOSFETs
. Electronics Letters
, 1994
. 30
(20
): p. 1634
–1635
.6.
Chaney
, A.
and R.
Sundararajan
, Simple MOSFET-based high-voltage nanosecond pulse circuit
. Plasma Science, IEEE Transactions
on, 2004
. 32
(5
): p. 1919
–1924
.7.
Gene Pulse/MicroPulser Electroporation Cuvette
.8.
Bio-Rad Laboratories: MicroPulser(TM) Electroporation Apparatus Operating Instructions and Applications Guide
.
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