Regulation of fluxes in a metabolic system aims to enhance the production rates of biotechnologically important compounds. Regulation is held via modification the cellular activities of a metabolic system. In this study, we present a metabolic analysis of ethanol fermentation process of a yeast cell in terms of continuous culture scheme. The metabolic regulation is based on the kinetic formulation in combination with metabolic control analysis to indicate the key enzymes which can be modified to enhance ethanol production. The model is used to calculate the intracellular fluxes in the central metabolism of the yeast cell. Optimal control is then applied to the kinetic model to find the optimal regulation for the fermentation system. The sensitivity results show that there are external and internal control parameters which are adjusted in enhancing ethanol production. As an external control parameter, glucose supply should be chosen in appropriate way such that the optimal ethanol production can be achieved. For the internal control parameter, we find three enzymes as regulation targets namely acetaldehyde dehydrogenase, pyruvate decarboxylase, and alcohol dehydrogenase which reside in the acetaldehyde branch. Among the three enzymes, however, only acetaldehyde dehydrogenase has a significant effect to obtain optimal ethanol production efficiently.
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9 March 2015
SYMPOSIUM ON BIOMATHEMATICS (SYMOMATH 2014)
31 August–2 September 2014
East Java, Indonesia
Research Article|
March 09 2015
Metabolic regulation and maximal reaction optimization in the central metabolism of a yeast cell
Kasbawati;
Kasbawati
Industrial & Financial Mathematics Research Group, Institut Teknologi Bandung, Bandung, Indonesia 40132 and Department of Mathematics, Hasanuddin University, Makassar,
Indonesia
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A. Y. Gunawan;
A. Y. Gunawan
Industrial & Financial Mathematics Research Group, Institut Teknologi Bandung, Bandung,
Indonesia
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R. Hertadi;
R. Hertadi
Biochemistry Research Division, Institut Teknologi Bandung, Bandung,
Indonesia
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K. A. Sidarto
K. A. Sidarto
Industrial & Financial Mathematics Research Group, Institut Teknologi Bandung, Bandung,
Indonesia
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Kasbawati
A. Y. Gunawan
R. Hertadi
K. A. Sidarto
Industrial & Financial Mathematics Research Group, Institut Teknologi Bandung, Bandung, Indonesia 40132 and Department of Mathematics, Hasanuddin University, Makassar,
Indonesia
AIP Conf. Proc. 1651, 75–85 (2015)
Citation
Kasbawati, A. Y. Gunawan, R. Hertadi, K. A. Sidarto; Metabolic regulation and maximal reaction optimization in the central metabolism of a yeast cell. AIP Conf. Proc. 9 March 2015; 1651 (1): 75–85. https://doi.org/10.1063/1.4914436
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