The aim of this study was to monitor the intracellular distribution of nucleic acids in human embryonic stem cells. Raman micro-spectroscopy and fluorescence imaging investigations were employed to obtain high-spatial resolution maps of nucleic acids. The DNA Raman signal was identified based on the 782 cm−1 band, while the RNA characteristic signal was detected based on the 813 cm−1 fingerprint band assigned to O-P-O symmetric stretching vibrations. Additionally, principal components analysis was performed and nucleic acids characteristic Raman signals were identified in the data set, which were plotted at each position in the cells. In this manner, high intensity RNA signal was identified in the cells nucleolus and cytoplasm, while the nucleus presented a much lower signal.
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13 November 2013
PROCESSES IN ISOTOPES AND MOLECULES (PIM 2013)
25–27 September 2013
Cluj Napoca, Romania
Research Article|
November 13 2013
Monitoring the RNA distribution in human embryonic stem cells using Raman micro-spectroscopy and fluorescence imaging
A. Falamas;
A. Falamas
National Institute for Research and Development of Isotopic and Molecular Technologies, 65-103 Donath, 400293, Cluj-Napoca, Romania and School of Physics and Astronomy, University of Nottingham, University Park, Nottingham, NG7 2RD,
United Kingdom
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S. Kalra;
S. Kalra
Wolfson Centre for Stem Cells, Tissue Engineering & Modeling, Centre for Biomolecular Sciences, University Park, University of Nottingham, NG7 2RD,
United Kingdom
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V. Chis;
V. Chis
Faculty of Physics, Babes-Bolyai University, 1 M. Kogalniceanu Street, 400084, Cluj-Napoca,
Romania
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I. Notingher
I. Notingher
School of Physics and Astronomy, University of Nottingham, University Park, Nottingham, NG7 2RD,
United Kingdom
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AIP Conf. Proc. 1565, 43–47 (2013)
Citation
A. Falamas, S. Kalra, V. Chis, I. Notingher; Monitoring the RNA distribution in human embryonic stem cells using Raman micro-spectroscopy and fluorescence imaging. AIP Conf. Proc. 13 November 2013; 1565 (1): 43–47. https://doi.org/10.1063/1.4833693
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