Policies to combat Chagas disease presents a considerable degree of negligence and is classified at level III by TDR, where the focus of research is based on the improvement and wider dissemination of existing tools and strategies for combating them. The PACT is based on topical or systemic administration of a nontoxic dye sensitive to light, followed by low dose irradiation with visible light of wavelength appropriate. In the presence of oxygen found in the cells, the photosensitizer (FS) enabled may react with molecules in its vicinity by electron transfer or hydrogen, leading to production of free radicals (type I reaction) or by energy transfer to oxygen (type II reaction), leading to production of singlet oxygen. Both paths can lead to cell death and the destruction of diseased tissue. In this work, we verify the effectiveness of PACT associated with a semiconductor laser InGaAlP, a wavelength (λ) equal to 30 mW optical power, emitting red light in the visible spectrum, with a dose of in continuous mode, using methylene blue in five differents concentrations on the infective trypomastigote forms of Trypanosoma cruzi. To determine the viability of the parasites, one sample from each treatment group at each concentration was removed and analyzed in a hemocytometer, observing the decrease in the number of live parasites for the solution without treatment. The results demonstrated significant percentage of parasite lysis (up to 86% lethality), what can not be observed in the groups treated with laser or with the FS.
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24 August 2011
ADVANCES IN LASEROLOGY ‐ SELECTED PAPERS OF LASER FLORENCE 2010: The 50th Birthday of Laser Medicine World
5–6 November 2010
Florence, (Italy)
Research Article|
August 24 2011
Evaluation of Photodynamic Antimicrobial Therapy (PACT) against Trypomastigotes of Trypanosoma cruzi: In Vitro Study
Artur F. S. Barbosa;
Artur F. S. Barbosa
aLaboratório de Planejamento e Síntese de Fármacos, Federal University of Pernambuco, 1235 Prof. Moraes Rego Ave, Cidade Universitária, Recife, PE, Brazil, 50670‐901
bCenter of Biophotonics, School of Dentistry, Federal University of Bahia, 62 Araujo Pinho Ave, Canela, Salvador, BA, Brazil, 40110‐150
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Luiz G. P. Soares;
Luiz G. P. Soares
bCenter of Biophotonics, School of Dentistry, Federal University of Bahia, 62 Araujo Pinho Ave, Canela, Salvador, BA, Brazil, 40110‐150
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Jouber M. S. Aciole;
Jouber M. S. Aciole
bCenter of Biophotonics, School of Dentistry, Federal University of Bahia, 62 Araujo Pinho Ave, Canela, Salvador, BA, Brazil, 40110‐150
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Gilberth T. S. Aciole;
Gilberth T. S. Aciole
bCenter of Biophotonics, School of Dentistry, Federal University of Bahia, 62 Araujo Pinho Ave, Canela, Salvador, BA, Brazil, 40110‐150
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Ivan R. Pitta;
Ivan R. Pitta
aLaboratório de Planejamento e Síntese de Fármacos, Federal University of Pernambuco, 1235 Prof. Moraes Rego Ave, Cidade Universitária, Recife, PE, Brazil, 50670‐901
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Suely L. Galdino;
Suely L. Galdino
aLaboratório de Planejamento e Síntese de Fármacos, Federal University of Pernambuco, 1235 Prof. Moraes Rego Ave, Cidade Universitária, Recife, PE, Brazil, 50670‐901
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Antonio L. B. Pinheiro
Antonio L. B. Pinheiro
bCenter of Biophotonics, School of Dentistry, Federal University of Bahia, 62 Araujo Pinho Ave, Canela, Salvador, BA, Brazil, 40110‐150
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AIP Conf. Proc. 1364, 55–59 (2011)
Citation
Artur F. S. Barbosa, Luiz G. P. Soares, Jouber M. S. Aciole, Gilberth T. S. Aciole, Ivan R. Pitta, Suely L. Galdino, Antonio L. B. Pinheiro; Evaluation of Photodynamic Antimicrobial Therapy (PACT) against Trypomastigotes of Trypanosoma cruzi: In Vitro Study. AIP Conf. Proc. 24 August 2011; 1364 (1): 55–59. https://doi.org/10.1063/1.3626912
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