The objectives of this in vitro study were to evaluate the apical sealing ability of experimental nano hydroxyapatite (HA)‐filled epoxy resin based endodontic sealer and to compare it with the commercial AH26 sealant. A total of 76 extracted human anterior teeth were instrumented using NiTi files and randomly divided into two groups of 33 teeth each and two control groups of 5 teeth each. The first group was obturated using gutta‐percha with AH26 sealer. The second group was obturated with the nano HA‐filled epoxy resin based sealer. All teeth were coated with nail polish except 2 mm from foramen apical and then suspended in 2% methylene blue for 7 days. All teeth were sectioned longitudinally for measuring penetration of the dye using stereo‐microscope (x36). The result showed that there was no statistically significant difference (p>0.05) in apical sealing ability between AH26 silver‐free sealer and nano HA sealer.

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