The present work describes a methodology that can be used for the creation of biomimetic membranes based in sequential assembling of biological molecules, to be used for radiation damage studies in an environment mimicking that of bio‐systems. The major features of the method, the so called layer‐by‐layer technique, as well as the experimental parameters to take into account for heterostructures production are summarized here. Results for radiation damage in DNA containing biomimetic membranes prepared from different conditions methods revealed that the radiation induced damage is dependent on the heterostructure molecular composition, proving that the environment is of extreme importance for the extent of radiation induced damage in biological molecules.

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