The present work describes a methodology that can be used for the creation of biomimetic membranes based in sequential assembling of biological molecules, to be used for radiation damage studies in an environment mimicking that of bio‐systems. The major features of the method, the so called layer‐by‐layer technique, as well as the experimental parameters to take into account for heterostructures production are summarized here. Results for radiation damage in DNA containing biomimetic membranes prepared from different conditions methods revealed that the radiation induced damage is dependent on the heterostructure molecular composition, proving that the environment is of extreme importance for the extent of radiation induced damage in biological molecules.
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8 December 2008
RADIATION DAMAGE IN BIOMOLECULAR SYSTEMS: Proceedings of the 5th International Conference (RADAM 2008)
13–15 June 2008
Debrecen (Hungary)
Research Article|
December 08 2008
Biomimetic Heterostructures for Radiation Damage Studies
M. Raposo;
M. Raposo
aCentro de Física e Investigação Tecnológica (CEFITEC), Departamento de Física, Faculdade de Ciências e Tecnologia, UNL, 2829‐516 Caparica, Portugal
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P. J. Gomes;
P. J. Gomes
aCentro de Física e Investigação Tecnológica (CEFITEC), Departamento de Física, Faculdade de Ciências e Tecnologia, UNL, 2829‐516 Caparica, Portugal
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J. M. C. Lourenço;
J. M. C. Lourenço
aCentro de Física e Investigação Tecnológica (CEFITEC), Departamento de Física, Faculdade de Ciências e Tecnologia, UNL, 2829‐516 Caparica, Portugal
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M. Coelho;
M. Coelho
aCentro de Física e Investigação Tecnológica (CEFITEC), Departamento de Física, Faculdade de Ciências e Tecnologia, UNL, 2829‐516 Caparica, Portugal
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S. V. Hoffmann;
S. V. Hoffmann
bInstitute for Storage Ring Facilities, University of Aarhus, Ny Munkegade, Building 1520, DK 8000 Aarhus C, Denmark
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A. M. Botelho do Rego;
A. M. Botelho do Rego
cCentro de Química‐Física Molecular, Complexo Interdisciplinar, Instituto Superior Técnico, 1049‐001 Lisboa, Portugal
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R. W. McCullough;
R. W. McCullough
dCentre for Plasma Physics, School of Mathematics and Physics, Queen’s University Belfast, University Road, Belfast BT7 1NN, Northern Ireland, UK
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N. J. Mason;
N. J. Mason
eDepartment of Physics and Astronomy, The Open University, Walton Hall, Milton Keynes MK7 6AA, UK
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C. Lage;
C. Lage
fLaboratório de Radiobiologia Molecular, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BR‐21949540, Brazil
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P. Limão‐Vieira;
P. Limão‐Vieira
aCentro de Física e Investigação Tecnológica (CEFITEC), Departamento de Física, Faculdade de Ciências e Tecnologia, UNL, 2829‐516 Caparica, Portugal
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P. A. Ribeiro
P. A. Ribeiro
aCentro de Física e Investigação Tecnológica (CEFITEC), Departamento de Física, Faculdade de Ciências e Tecnologia, UNL, 2829‐516 Caparica, Portugal
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M. Raposo
a
P. J. Gomes
a
J. M. C. Lourenço
a
M. Coelho
a
S. V. Hoffmann
b
A. M. Botelho do Rego
c
R. W. McCullough
d
N. J. Mason
e
C. Lage
f
P. Limão‐Vieira
a
P. A. Ribeiro
a
aCentro de Física e Investigação Tecnológica (CEFITEC), Departamento de Física, Faculdade de Ciências e Tecnologia, UNL, 2829‐516 Caparica, Portugal
bInstitute for Storage Ring Facilities, University of Aarhus, Ny Munkegade, Building 1520, DK 8000 Aarhus C, Denmark
cCentro de Química‐Física Molecular, Complexo Interdisciplinar, Instituto Superior Técnico, 1049‐001 Lisboa, Portugal
dCentre for Plasma Physics, School of Mathematics and Physics, Queen’s University Belfast, University Road, Belfast BT7 1NN, Northern Ireland, UK
eDepartment of Physics and Astronomy, The Open University, Walton Hall, Milton Keynes MK7 6AA, UK
fLaboratório de Radiobiologia Molecular, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BR‐21949540, Brazil
AIP Conf. Proc. 1080, 125–131 (2008)
Citation
M. Raposo, P. J. Gomes, J. M. C. Lourenço, M. Coelho, S. V. Hoffmann, A. M. Botelho do Rego, R. W. McCullough, N. J. Mason, C. Lage, P. Limão‐Vieira, P. A. Ribeiro; Biomimetic Heterostructures for Radiation Damage Studies. AIP Conf. Proc. 8 December 2008; 1080 (1): 125–131. https://doi.org/10.1063/1.3058971
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